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Fast-scan cyclic voltammetry (FSCV) is cyclic voltammetry with a very high scan rate (up to 1×106 V·s−1). Application of high scan rate allows rapid acquisition of a voltammogram within several milliseconds and ensures high temporal resolution of this electroanalytical technique. An acquisition rate of 10 Hz is routinely employed.
FSCV in combination with carbon-fiber microelectrodes became a very popular method for detection of neurotransmitters, hormones and metabolites in biological systems. Initially, FSCV was successfully used for detection of electrochemically active biogenic amines release in chromaffin cells (adrenaline and noradrenaline), brain slices (5-HT, dopamine, norepinephrine) and in vivo in anesthetized or awake and behaving animals (dopamine). Further refinements of the method have enabled detection of 5-HT, HA, norepinephrine, adenosine, oxygen, pH changes in vivo in rats and mice as well as measurement of dopamine and serotonin concentration in fruit flies.
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